The Effect of Basic and Acidic Additives on the Separation of -Blocker Enantiomers on Polysaccharide-based Chiral Columns with Acetonitrile as Mobile Phase

Author: Khatuna Gogaladze
Co-authors: Khatuna Gogaladze, Bezhan Chankvetadze, Lali Chankvetadze
Keywords: Beta-Blockers, HPLC, enantiomers
Annotation:

The separation of enantiomers of 16 -blocker drugs was studied using polysaccharide-based chiral selectors and acetonitrile as mobile phase with emphasis on the role of basic and acidic additives on separation and elution order of enantiomers. Out of the studied chiral selectors, amylose phenylcarbamate-based ones showed more often chiral recognition ability compared to cellulose phenylcarbamate derivatives. An interesting effect was observed with formic acid as additive on enantiomer resolution and enantiomer elution order for some -blocker drugs. Thus, for instance, the enantioseparation of several -blockers (atenolol, sotalol, toliprolol; all basic compounds) improved not only by the addition of a more conventional basic additive to the mobile phase, but also by the addition of an acidic additive. Moreover, opposite elution order of enantiomers was observed depending on the nature of additive (basic or acidic) in the mobile phase.