The Biophycal research regarding the compounds made of Dendrimer G4 and its various ligands

Author: mariam tsitsvidze
Keywords: Dendrimer, Nanoparticle, SDS
Annotation:

Annotation. The present paper deals with PAMAM G4 DENDRIMER, SDS Detergents, salt and DNA - it's a complex calorimetric studies. The purpose of this paper is credible, which means in this complex, as "necessary" transporter gene in a cell to produce nanoparticles. In order to Achieving linked DENDRIMER - DNA - the temperature stability of the complex Determination, as is necessary to know the complex structure physical - chemical properties. The experiments, based on the particular obtained from the calorimetric curves, it is clear that there is DNA - it thermal melting curve shape change after the DNA - the molecule forming ties with the G4 DENDRIMER. The emergence of these connections is confirmed heat absorption calorimetric curves change (pure DNA - it Curves of heat absorption), which proves that arose Complex (there are ties) DNA - and the G4 DENDRIMER. On the other hand, also It is clear that DENDRIMER predominantly associated with DNA - the molecule of the Districts, which are rich in guanine - cytosine pair.Very useful out to be a triple complex consisting of DNA - found, PAMAM G4 DENDRIMER SDS and salt it. SDS detergents, salt is needed to In order for that to happen, and the DNA molecule of PAMAM G4 DENDRIMER the nozzles the negative charge of the phosphate shielded, thus obtained The nanoparticles are more efficient gene transfer. It is shown that SDS salt DNA - The molecule has formed a mixed complex, which increases the DNA - it thermally, which also depends on the SDS the concentration of the of salt. DNA - the SDS solutions adding salt and PAMAM G4 DENDRIMER place DNA the molecule on the stability of two different structures Formation.